My Full Longevity Protocol (2026): Supplements, Testing, and What I'm Actually Taking

Everything I take, why I take it, what I test, and what it costs. No mystery, no gatekeeping.

I've been tinkering with longevity supplements for about two years. I started with standalone NMN and resveratrol, added TMG after learning about the methylation problem, then gradually accumulated a shelf full of bottles. At peak complexity I was managing 12 separate supplements across morning and evening doses.

When DoNotAge launched the sachet, I consolidated. My protocol went from 12 bottles to one sachet plus two capsules. Here's what the current stack looks like, what it costs, and why I chose each component.

Morning stack

DoNotAge daily sachet mixed in water. 15 ingredients covering NAD+ production (NMN, resveratrol, SIRT6 Activator, TMG, Ca-AKG), cellular cleanup (spermidine, quercetin, fisetin, sulforaphane), and vascular support (D3, K2, magnesium, CoQ10, Nitralis). I take this first thing with breakfast.

Vitamin D3 at 5,000 IU (separate capsule). The sachet's D3 dose is almost certainly below 5,000 IU, and I'm targeting serum levels of 60 to 80 ng/mL. My last blood test showed 52 ng/mL, so I have room to push higher. I take this with breakfast because D3 is fat-soluble and absorbs better with dietary fat.

See also: Vitamin D optimization: why 600 IU is not enough

Evening stack

Magnesium glycinate at 300 mg. This fills the magnesium gap left by the sachet's L-Threonate (which is great for the brain but delivers negligible elemental magnesium). Glycinate has a calming effect that helps with sleep quality. I take it about an hour before bed.

See also: Magnesium L-Threonate vs. glycinate vs. citrate vs. oxide

What I'm not taking (and why)

Berberine. I've seen the glucose-lowering data and the AMPK activation research. My fasting glucose and HbA1c are both in range, so the metabolic benefit is marginal for me. If either marker drifted, I'd add it.

Rapamycin. The mTOR inhibition data for longevity is fascinating but the risk profile for off-label use doesn't sit right with me yet. I'm watching the clinical trials.

Extra omega-3. My diet includes enough fatty fish (salmon, sardines) that supplemental fish oil feels redundant. If I ate less fish, I'd add 2 g of EPA/DHA.

Metformin. Same reasoning as berberine. My metabolic markers don't warrant it, and the TAME trial results aren't published yet.

Monthly cost breakdown

That's $7.75 per day for a protocol covering 15 longevity compounds plus the D3 and magnesium gaps. Building the equivalent from individual bottles would run $700+ per month.

See also: DoNotAge sachet pricing: what $7.25 per day actually gets you

Lab testing schedule

I test twice a year for the full panel and more frequently for specific markers when I'm evaluating a new intervention.

Core panel (every 6 months): NAD+ (Jinfiniti intracellular test), fasting glucose, HbA1c, fasting insulin, HOMA-IR, full lipid panel including ApoB, hs-CRP, homocysteine, liver enzymes (ALT, AST, GGT), 25-hydroxyvitamin D, RBC magnesium, kidney function (creatinine, BUN, eGFR), total and free testosterone, DHEA-S, IGF-1, CBC with differential.

NAD+ tracking (at protocol changes): Baseline before starting, 30 days, 60 days. After that, every 6 months is enough.

Optional but valuable: Epigenetic biological age test (TruDiagnostic or GrimAge) annually. These run $200 to $400 per test but give you the most comprehensive single measure of whether your protocol is actually slowing biological aging.

See also: How to test your NAD+ levels at home

What I'm watching for in my labs

Homocysteine trending down would confirm the TMG in the sachet is doing its job as a methyl donor.

hs-CRP staying below 1.0 would indicate that the anti-inflammatory compounds (quercetin, sulforaphane) and senolytic effects (fisetin) are managing systemic inflammation.

NAD+ increasing 40 to 80% from baseline would confirm the NMN is absorbing despite the non-liposomal delivery.

Vitamin D reaching 60 to 80 ng/mL would validate my supplemental D3 dose on top of the sachet.

What I'd change at different budgets

$100/month: NOVOS Core at $79 to $99. Fewer ingredients but solid coverage of the main aging pathways. Add D3 ($2/month) and magnesium ($12/month). Total around $113.

$200/month: DoNotAge sachet at $203 plus D3 and magnesium. This is what I'm doing.

$300/month: DoNotAge sachet plus standalone fisetin for monthly high-dose senolytic protocol (500 mg for 2 days, once monthly). Add a biological age test annually. Possibly add ubiquinol CoQ10 at 200 mg if you want to ensure the CoQ10 dose is at clinical levels.

See also: Best longevity supplement stacks ranked (2026)

Frequently asked questions